Elevation of Bombina variegata peptide 8 in mice with collagen-induced arthritis

نویسندگان

  • Daitaro Kurosaka
  • Kentaro Noda
  • Ken Yoshida
  • Kazuhiro Furuya
  • Taro Ukichi
  • Eigo Takahashi
  • Maimi Yanagimachi
  • Isamu Kingetsu
  • Saburo Saito
  • Akio Yamada
چکیده

BACKGROUND Bombina variegate peptide 8 (Bv8) is a small protein secreted by frog skin. Recently it has been shown to contribute to tumor angiogenesis in mouse model. The purpose of this study was to investigate Bv8 in mice with type II collagen-induced arthritis (CIA). METHODS We induced CIA in male DBA/1J mice. The severity of arthritis was evaluated based on an arthritis score. RNA was extracted from the joint, and examined for Bv8 mRNA expression by RT-PCR and real-time RT-PCR. Synovial tissue and bone marrow were immunohistochemically examined using anti-Bv8 antibody. RESULTS The level of Bv8 mRNA expression in the joint was below the detection limit in the control group, but was elevated in the CIA group, and was correlated with the arthritis score. In addition, an increase in Bv8-positive cells was observed in the synovium and bone marrow in the CIA group. CONCLUSION Bv8 was elevated in the synovium and bone marrow of CIA mice, suggesting that Bv8 plays an important role in the pathogenesis of arthritis.

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Author's response to reviews Title: Elevation of Bombina variegata peptide 8 in mice with collagen-induced arthritis Authors:

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عنوان ژورنال:
  • BMC Musculoskeletal Disorders

دوره 10  شماره 

صفحات  -

تاریخ انتشار 2009